Isabel Moraes

• College Algebra
• Environmental Science
• Calculus with Applications
• Elementary Statistics
• Physics
• Human Biology

• Biochemistry
• Maths and Physics
• Human health

My research is focused on the development of structural biology capabilities using X-ray crystallography, X-ray Free Electron Lasers, Cryo-Electron Microscopy and other biophysical approaches to establish a comprehensive measurement system that will provide metrological foundations for structure-function relationships of macrobiomolecules (proteins and nucleic acids). This will enable exploitation of properties and functionalities in a variety of biomedical contexts including drug attrition, antimicrobial discovery, synthetic biology and cellular therapies.

Publications

J. F.B. Juarez et al. & Moraes, I. Internal water networks underpin receptor sensitisation: high-resolution structures of dark- and light-adapted H+ transporter. 2020

T. O. Kwan, Reis, R., & Moraes, I. In Situ Measurements of Polypeptide Samples by Dynamic Light ScatteringDynamic light scattering (DLS): Membrane Proteins, a Case Study. 2020. Peptide Nanomaterials: Methods and Protocols, Springer

A. Butryn, H. Raza, H. Rada, I. Moraes, R. J. Owens, and A. M. Orville. Molecular basis for GTP recognition by light‐activated guanylate cyclase RhGC. The FEBS Journal  2019.

A. Ishchenko, B. Stauch, G. Won Han, A. Batyuk, A. Shiriaeva, C. Li, N. Zatsepin, U. Weierstall, W. Liu, E. Nango, T. Nakane, R. Tanaka, K. Tono, Y. Joti, S. Iwata, I. Moraes, C. Gatil and V. Cherezov. Toward G protein-coupled receptor structure-based drug design using X-ray lasers.  IUCrJ, 2019 6(6).

T.O. Kwan, Reis, R., Siligardi, G., Hussain, R., Cheruvara, H., & Moraes, I. Selection of Biophysical Methods for Characterisation of Membrane Proteins. International journal of molecular sciences 2019, 20:2605.

R. Reis & I. Moraes, Structural biology and structure–function relationships of membrane proteins. Biochemical Society Transactions 2019, 47:47-61.
R. S.K. Nagampalli, J.E.N. Quesñay, Z. Islam, James Birch, H.G. Sebinelli, C.F.R. Ascenção, D. Adamoski, A.M.Fala, B.A. Pauletti, S.R. Consonni, A.C.T. Silva, J. F. Oliveira, K.G. Franchini, A.F. P. Leme, P. Ciancaglini, I. Moraes, S.M.G. Dias & A.L.B. Ambrosio. Human mitochondrial pyruvate carrier 2 is a homodimeric autonomous transporter in vitro. Scientific Reports 2018, 8:3510.

J. Birch, D. Axford, J. Foadi, A. Meyere, A. Eckardte, Y. Thielmannf, I. Moraes. The fine art of membrane protein crystallization. METHODS 2018, 147:150-162.
A. Mylona, S. Carr, S., P. Aller, I. Moraes, R. Treisman, G. Evans, & J. Foadi. A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software. Crystals 2017,7:242.

A. Anandhi, G.L. Evans, K. Condic-Jurkic, M.L. O’Mara, C.M. John, N. J. Phillips, G.A. Jarvis, S. S. Wills, K. A. Stubbs, I. Moraes, C.M. Kahler and A. Vrielink. Structure of a lipid A phosphoethanolamine transferase suggests how conformational changes govern substrate binding. Proceedings of the National Academy of Sciences 2017, 114: 2218-2223.

I. Moraes (ed.); The Next Generation in Membrane Protein Structure Determination; Springer; Volume 922 of the series Advances in Experimental Medicine and Biology – 2016

J. Sanchez-Weatherby and I. Moraes; Crystal Dehydration in Membrane Protein Crystallography; The Next Generation in Membrane Protein Structure Determination. Springer; Volume 922 of the series Advances in Experimental Medicine and Biology 2016, pp73-89.

A. Heifetz, G.F.X. Schertler, R. Seifert, C.G. Tate, P.M. Sexton, V.V. Gurevich, D. Fourmy, V. Cherezov, F. H. Marshall, R.I. Storer, I. Moraes, I.G. Tikhonova, C.S. Tautermann, P. Hunt, T. Ceska, S. Hodgson, M.J. Bodkin, S. Singh, R.J. Law and P. C. Biggin. GPCR Structure, Function, Drug Discovery and Crystallography: Report from Academia-Industry International Conference (UK Royal Society) Chicheley Hall, 1-2 September 2014. Naunyn-Schmiedeberg’s Archives of Pharmacology   2015, 388:8

P. Nogly, D. James, D. Wang, T. White, N. Zatsepin, A. Shilova, G. Nelson, H. Liu, L. Johansson, M. Heymann, K. Jäger, M. Metz, W. Wu, P. Baath, P.Berntsen, , D. Oberthuer, V. Panneels, V. Cherezov, H. Chapman, R. Neutze, J. Spence, G. Schertler, M. Burghammer,, I. Moraes, J. Standfuss, U. Weierstall, Lipidic cubic phase serial millisecond crystallography using synchrotron radiation.   IUCrJ  2015, 2:168-176.

P. Nogly, I. Gushchin, A. Remeeva, A.M. Esteves, A. Ishchenko, P. Ma, S. Grudinin, N. Borges, E Round, I. Moraes, V. Borshchevskiy, H. Santos, V. Gordeliy & M. Archer.  X-ray structure of a membrane protein from the CDP-alcohol phosphatidyltransferase family.  Nature Communications 2014, 5:4169

L.E. Bird, H. Rada, A. Verma, R. Gasper, J. Birch, M. Jennions, J. Lӧwe, I. Moraes and R.J. Owens. Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli. J.Vis. Exp  2014, 67:e52357

A. Sverzhinsky, S. Qian, L. Yang, M. Allaire, I. Moraes, D. Ma, J. W. Chung, M. Zoonens, J-L. Popot and J. W. Coulton. Amphipol-trapped ExbB−ExbD Membrane Protein Complex from Escherichia coli: a Biochemical and Structural Case Study. Journal of Membrane Biology 2014, 1-14.

I. Moraes & Margarida Archer, Successful Membrane Protein Crystallization, Structural Proteomics – Methods in Molecular Biology 2nd edition, Editor: Ray Owens, Springer 2014

I. Moraes, G. Evans J. Sanchez-Weatherby, S. Newstead, Patrick Shawn. Structure determination of membrane proteins – The next generation. Biochimica et Biophysica Acta Biomembranes 2014,1838:78-87.

A. Douangamath, P. Aller, P. Lukacik,J. Sanchez-Weatherby, I. Moraes and J. Brandao-Neto. Using high-throughput in situ plate screening to evaluate the effect of dehydration on protein crystals. Acta Cryst. 2013, D69, 920-923.

D. Axford, R.  L. Owen, J. Foadi, A. W. Morgan, James I. Robinson, J. Nettleship, R. Owens, I. Moraes, D. I. Stuart, J. Ren, E. E. Fry, K. Harlos, A. Kotecha and G.  Evans. In situ macromolecular crystallography using microbeams. Acta Cryst. 2012, D68, 592-600.

M. Smith, V. Mack, A. Ebneth, I. Moraes, B. Felicetti, M. Wood, D. Schonfeld, O. Mather, A. Cesura, J. Barker. The Structure of Mammalian Serine Racemase. Evidence for Conformational Changes Upon Inhibitor Binding.  J Biol Chem., 2010, 285:12873–12881.

• Member of the British Crystallography Association
• Member of the International Union of crystallography
• Member of the British Biophysical Society
• Governor of Newbold College